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20-May-2019 00:27

Lysates from transfected cells were immunoprecipitated using STT3B antibody or control Ig G and TUSC3 was detected using western blot.Ig G light chain (Ig G LC) serves as loading control.N-glycosylation of the wild type firefly luciferase in HEK293T cells leads to a change in molecular weight in SDS-PAGE (Supplementary Figure S1a) and decreased enzymatic activity (Figure 1c).

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(d) HEK293T cells were co-transfected with ER-Luc and empty pc DNA 3.1 vector (V) or full length TUSC3.These patients constitute a heavily pretreated and highly heterogeneous population and despite current advances in treatment of CRPC patients, the 5-year survival rate remains less than 5%.